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Thursday, July 9, 2020 | History

4 edition of Luminescent assays found in the catalog.

Luminescent assays

perspectives in endocrinology and clinical chemistry

  • 375 Want to read
  • 27 Currently reading

Published by Raven Press in New York .
Written in English

    Subjects:
  • Hormones -- Analysis -- Congresses.,
  • Fluorimetry -- Congresses.,
  • Clinical chemistry -- Technique -- Congresses.

  • About the Edition

    Summary of lectures presented at the Meeting on Luminescent Assays: Perspectives in Endocrinology and Clinical Chemistry, held in Florence, July 1981, organized by the Post-Graduate School of Endocrinology, University of Florence.

    Edition Notes

    Includes bibliographies and index.

    Statementeditors, Mario Serio, Mario Pazzagli.
    SeriesSerono symposia publications from Raven Press ;, v. 1
    ContributionsSerio, Mario., Pazzagli, Mario., Università di Firenze. Scuola di endocrinologia., Meeting on Luminescent Assays: Perspectives in Endocrinology and Clinical Chemistry (1981 : Florence, Italy)
    Classifications
    LC ClassificationsRB48.5 .L8 1982
    The Physical Object
    Paginationxv, 286 p. :
    Number of Pages286
    ID Numbers
    Open LibraryOL3783974M
    ISBN 100890047405
    LC Control Number81040363

    dead cells (cytotoxicity assay), the number of live cells (viability assay), the total number of cells or the mechanism of cell death (e.g., apoptosis). Table compares Promega homogeneous cell-based assays and lists the measured parameters, sensitivity of detection, incubation time and detection method for each assay. The Kinase-Glo® Assays are performed in a single well of a multiwell plate by adding a volume of Kinase-Glo® Reagent equal to the volume of a completed kinase reaction and measuring luminescence. The luminescent signal is correlated with the amount of ATP present and is inversely correlated with the amount of kinase activity. Summary of Changes.

    To assay the effect of the compounds on the CP Luc/42AA, 20 μL of the CP Luc/42AA mixture was added to all wells in one set of four plates. To the second set of four plates, 5 μL ProTEV protease diluted to U/μL in 10 mM HEPES, pH was added, except for the no enzyme control lanes which received only dilution by: Chemiluminescence (also chemoluminescence) is the emission of light (luminescence), as the result of a chemical may also be limited emission of heat. Given reactants A and B, with an excited intermediate, [A] + [B] → [ ] → [Products] + lightFor example, if [A] is luminol and [B] is hydrogen peroxide in the presence of a suitable catalyst we have.

    Bioluminescence Methods and Protocols represents the state-of-the-art in luminescent assays. Its cutting-edge techniques, diversely illustrated applications, and detailed instructions will make it the standard reference for today's investigators who want to adapt established techniques to their purposes or apply them to a wide range of clinical. Protein-protein interaction assays are fundamental to basic biology, drug discovery, diagnostics, screening, and immunoassays. Protein-fragment complementation (PCA) is one of such useful protein-protein interaction assays. PCA when performed using luciferase is a reversible approach, whereas when performed using green fluorescent protein analogs is an irreversible : Yuki Ohmuro-Matsuyama, Hiroshi Ueda.


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Luminescent assays Download PDF EPUB FB2

The Kinase-Glo Luminescent Kinase Assays are homogeneous non-radioactive methods for determining the activity of purified kinases by quantifying the amount of ATP remaining in solution following a kinase reaction.

A Luminescent Assay to Determine the Number of Viable Cells in Culture A homogeneous "add-mix-measure" format reduces the number of steps Higher level of detection requires fewer cells per assay. Coupled Bioluminescent Assays, explains the uses of CB technologies across drug discovery to analyze toxicity, drug receptors, and enzymes.

It covers applications in environmental analysis and biodefense, including cytotoxicity, fertilizer and explosives analysis, and nerve agent and pesticide detection. The rapid decrease in luminescent signal following cell death enables multiplexing this assay with other luminescent assays that contain a lysis step that will kill cells.

The decrease in luminescence following cell death is important to eliminate interference with subsequent luminescent assays [54, 60].Cited by:   The book presents contemporary key topics like luminescent marine organisms, DNA probes, reporter gene assays and photoproteins, ratiometric sensing, use of photoproteins for in vivo functional imaging and luminescent proteins in binding assays, to name just a few, and is complemented by recent advances in instrumentation.

luminescence has maintained a prominent position among certain assay formats, most notably genetic reporters.

Recently, there has been growing partiality for luminescent assays across a wider range of applications due to their sensitivity, broad linearity, and robustness to library compounds and complex biological samples. These assays do not enable an exploration beyond a simple analysis, so we set out to modify SecYEG PLs, IMVs and substrate pre-proteins such that they would be compatible with a split NanoLuc-based assay (Figs.

1b and S1b).For PLs, we included purified 6H 11S into the reconstitution mixture and then removed excess protein by three rounds of centrifugation and resuspension in clean buffer, to Cited by: 4.

The assay methods covered include the use of different classes of colorimetric tetrazolium reagents, resazurin reduction and protease substrates generating a fluorescent signal, the luminogenic ATP assay, and a novel real-time assay to monitor live cells for days in by: numbers shown.

The cells were assayed using CellTiter 96® (Promega; MTS assay) and ViaLight® Plus Kit (n=4.). As the cell number decreases, the MTS Assay loses sensitivity and only detects >5, cells/well. The linearity of this MTS Assay seems to be betweencells/well in this model.

ViaLight® Plus shows. All assays were performed in black well plate and in 50 μL final volume using triplicates. CP-peptide binding tests were performed with PKA substrate peptides (CP), by utilizing previously introduced Eu 3+-LZ reporter peptide in readout [].All used peptides are listed in Table assays were performed in assay buffer containing 10 mM HEPES (pH ), mM EDTA, % Triton, 5 mM Author: Kari Kopra, Natalia Tong-Ochoa, Mari Laine, Ville Eskonen, Päivi J.

Koskinen, Harri Härmä. This is a PDF-only article. The first page of the PDF of this article appears by: Luminescent ATP Detection Assay Kit (ab) allows the detection of total levels of cellular ATP (adenosine triphosphate). The ATP assay is based on the production of light caused by the reaction of ATP with added firefly’s luciferase and Size: KB.

This book highlights the applications of coupled bioluminescence assay techniques to real-world problems in drug discovery, environmental and chemical analysis, and biodefense.

It separates theoretical aspects from the applied sections in a clear and readable way. Coupled Bioluminescent Assays, explains the uses of CB technologies across drug discovery to analyze toxicity, drug Author: Michael J. Corey. Luminescence is a generic term that covers a range of processes which produce light.

Two reactions, chemiluminescence and bioluminescence, are increasingly being used to monitor immunoassays (1). Luminescent oxygen channeling assay (LOCI) is a homogeneous immunoassay method capable of rapid, quantitative determination of a wide range of analytes--including high and very low concentrations.

There is a linear relationship (r 2 = ) between the luminescent signal and the number of cells from 0 to 50, cells per well in assays performed in a 96 well format. Get this from a library.

Luminescent assays: perspectives in endocrinology and clinical chemistry. [Mario Serio; Mario Pazzagli; Università di Firenze.

Scuola di endocrinologia.;] -- Summary of lectures presented at the Meeting on Luminescent Assays: Perspectives in Endocrinology and Clinical Chemistry, held in Florence, Julyorganized by the Post-Graduate School of. Luminescent labels have been widely used for biological applications, primarily in bioimaging and assays.

Recently much attention has been focused on the development of luminescent nanoprobes with excitation/emission maxima falling in the region of minimal tissue absorbance/autofluorescence between and nm, an “imaging window”.

PART I BACKGROUND TO COUPLED BIOLUMINESCENT ASSAYS 1 1 Introduction 3. Introduction to Coupled Bioluminescent Assays, 3 Luminescent Technologies of the Life Sciences, 4 Varieties of Fluorometric Assays, 7 Time-Resolved Fluorescence, 8 Fluorescence Resonance Energy Transfer, 8 Fluorescence Polarization, 9.

Abstract: In the development of high throughput screening (HTS) as a central paradigm of drug discovery, fluorescence has generally been adopted as the favored methodology.

Nevertheless, luminescence has maintained a prominent position among certain assay formats, most notably genetic reporters. Recently, there has been growing partiality for luminescent assays across a wider range of Cited by:.

Herein we report the first use of transition metal catalytic signal enhancement for the analysis of small organic analytes. Two assays using Sonogashira and Suzuki cross-couplings have been used in the detection of ketones and aldehydes produce highly luminescent markers.

The latter analysis utilizing the Su In honour of George Gokel.Ligand-sensitized, luminescent lanthanide(III) complexes are of considerable importance because their unique photophysical properties (microsecond to millisecond lifetimes, characteristic and narrow emission bands, and large Stokes shifts) make them well suited as labels in fluorescence-based bioassays.

The long-lived emission of lanthanide(III) cations can be temporally resolved from Cited by: Luminescence-based protein complementation assay. (A) The principle of the luminescent complementation assay is shown schematically.

(B) Split versions of the luminescent reporters Firefly, Renilla, and Nanoluciferase are shown in blue and red.

Purple refers to overlapping by: 3.